Elucidating protein dynamics and function using fluorescence correlation spectroscopy (FCS)
Part 3. Proteomics
3.4. Functional Proteomics
Short Specialist Review
Published Online: 15 APR 2005
Copyright © 2005 John Wiley & Sons, Ltd
Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics
How to Cite
Schwille, P. 2005. Elucidating protein dynamics and function using fluorescence correlation spectroscopy (FCS). Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.4:56.
- Published Online: 15 APR 2005
The principles and most prominent recent applications of fluorescence correlation spectroscopy (FCS) are outlined, with special focus on intracellular studies, particularly protein dynamics and interactions in situ. FCS is a very valuable alternative to other fluorescence-based techniques such as FRET, due to its extreme sensitivity that can readily reach the single-molecule level, and due to its high temporal resolution that allows us to study molecular dynamics on millisecond to microsecond scales.
- single molecule;
- protein–protein interaction;
- two-photon excitation;