Standard Article

Elucidating protein dynamics and function using fluorescence correlation spectroscopy (FCS)

Part 3. Proteomics

3.4. Functional Proteomics

Short Specialist Review

  1. Petra Schwille

Published Online: 15 APR 2005

DOI: 10.1002/047001153X.g304311

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Schwille, P. 2005. Elucidating protein dynamics and function using fluorescence correlation spectroscopy (FCS). Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.4:56.

Author Information

  1. Dresden University of Technology, Dresden, Germany

Publication History

  1. Published Online: 15 APR 2005

Abstract

The principles and most prominent recent applications of fluorescence correlation spectroscopy (FCS) are outlined, with special focus on intracellular studies, particularly protein dynamics and interactions in situ. FCS is a very valuable alternative to other fluorescence-based techniques such as FRET, due to its extreme sensitivity that can readily reach the single-molecule level, and due to its high temporal resolution that allows us to study molecular dynamics on millisecond to microsecond scales.

Keywords:

  • FCS;
  • single molecule;
  • cross-correlation;
  • protein–protein interaction;
  • diffusion;
  • endocytosis;
  • two-photon excitation;
  • FRET;
  • GFP;
  • TIR