Imaging protein function using fluorescence lifetime imaging microscopy (FLIM)
Part 3. Proteomics
3.4. Functional Proteomics
Short Specialist Review
Published Online: 15 JUL 2005
Copyright © 2005 John Wiley & Sons, Ltd
Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics
How to Cite
Gu, Y. and Zicha, D. 2005. Imaging protein function using fluorescence lifetime imaging microscopy (FLIM). Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.4:55.
- Published Online: 15 JUL 2005
Fluorescence lifetime has been used in various protein function studies in both fixed and living cells, including contrast enhancement, colocalization, and interactions. In this review, current FLIM (fluorescence lifetime imaging microscopy) technologies and data processing methodologies are briefly summarized. Special attention is paid to the lifetime retrieval of multicomponent complexes, where the combination of confocal-based time-domain FLIM with spectral unmixing technique can provide more accurate estimation. Typical examples of protein function interpretations using FLIM are also discussed.
- spectral unmixing;
- laser scanning confocal microscopy;