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Imaging protein function using fluorescence lifetime imaging microscopy (FLIM)

Part 3. Proteomics

3.4. Functional Proteomics

Short Specialist Review

  1. Yan Gu,
  2. Daniel Zicha

Published Online: 15 JUL 2005

DOI: 10.1002/047001153X.g304316

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Gu, Y. and Zicha, D. 2005. Imaging protein function using fluorescence lifetime imaging microscopy (FLIM). Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.4:55.

Author Information

  1. Cancer Research UK London Research Institute, London, UK

Publication History

  1. Published Online: 15 JUL 2005

Abstract

Fluorescence lifetime has been used in various protein function studies in both fixed and living cells, including contrast enhancement, colocalization, and interactions. In this review, current FLIM (fluorescence lifetime imaging microscopy) technologies and data processing methodologies are briefly summarized. Special attention is paid to the lifetime retrieval of multicomponent complexes, where the combination of confocal-based time-domain FLIM with spectral unmixing technique can provide more accurate estimation. Typical examples of protein function interpretations using FLIM are also discussed.

Keywords:

  • FRET;
  • spectral unmixing;
  • laser scanning confocal microscopy;
  • TCSPC