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Immunofluorescent labeling and fluorescent dyes

Part 3. Proteomics

3.4. Functional Proteomics

Basic Techniques and Approaches

  1. Peter Watson,
  2. Krysten J. Palmer,
  3. David J. Stephens

Published Online: 15 JAN 2005

DOI: 10.1002/047001153X.g304412

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Watson, P., Palmer, K. J. and Stephens, D. J. 2005. Immunofluorescent labeling and fluorescent dyes. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.4:58.

Author Information

  1. University of Bristol, Bristol, UK

Publication History

  1. Published Online: 15 JAN 2005

Abstract

This article sets out to cover some of the key points to consider when processing living cells and tissues for immunofluorescence. We discuss approaches to fix and image cells following immunolabeling, including using high-throughput screening technologies. We review the production and relative merits of different antibodies and compare different fluorophores, including recently developed reagents such as quantum dots. We also consider the use of recombinant antibody technology and the correlation of images obtained first by immunofluorescence microscopy at low resolution (e.g., confocal), and higher resolution ultrastructural analyses by electron microscopy.

Keywords:

  • immunofluorescence;
  • fixation;
  • fluorophore;
  • antibody