Immunofluorescent labeling and fluorescent dyes
Part 3. Proteomics
3.4. Functional Proteomics
Basic Techniques and Approaches
Published Online: 15 JAN 2005
Copyright © 2005 John Wiley & Sons, Ltd
Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics
How to Cite
Watson, P., Palmer, K. J. and Stephens, D. J. 2005. Immunofluorescent labeling and fluorescent dyes. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.4:58.
- Published Online: 15 JAN 2005
This article sets out to cover some of the key points to consider when processing living cells and tissues for immunofluorescence. We discuss approaches to fix and image cells following immunolabeling, including using high-throughput screening technologies. We review the production and relative merits of different antibodies and compare different fluorophores, including recently developed reagents such as quantum dots. We also consider the use of recombinant antibody technology and the correlation of images obtained first by immunofluorescence microscopy at low resolution (e.g., confocal), and higher resolution ultrastructural analyses by electron microscopy.