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Protein phosphorylation analysis – a primer

Part 3. Proteomics

3.5. Proteome Diversity

Basic Techniques and Approaches

  1. Judith A. Jebanathirajah1,2,
  2. Hanno Steen3

Published Online: 15 APR 2005

DOI: 10.1002/047001153X.g305411

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Jebanathirajah, J. A. and Steen, H. 2005. Protein phosphorylation analysis – a primer. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.5:73.

Author Information

  1. 1

    Harvard Medical School, Boston, MA, USA

  2. 2

    MDS Sciex, Concord, ON, Canada

  3. 3

    Children's Hospital and Harvard Medical School, Boston, MA, USA

Publication History

  1. Published Online: 15 APR 2005

Abstract

Protein phosphorylation is possibly the most common intracellular protein modification affecting several different amino acid residues. Its dynamic nature makes it extremely important in cellular regulation mechanisms warranting the need for reliable, fast, sensitive methods for the detection of this modification. The primer gives a brief synopsis on the method that is most commonly used in today's biological laboratories with references to the original literature for more detailed reading.

Keywords:

  • radioactive labeling;
  • Edman degradation;
  • phosphospecific antibodies;
  • mass spectrometry;
  • IMAC;
  • precursor ion scanning;
  • neutral loss scanning