Standard Article

Mass spectrometry

Part 3. Proteomics

3.5. Proteome Diversity

Basic Techniques and Approaches

  1. David J. Harvey

Published Online: 15 APR 2005

DOI: 10.1002/047001153X.g305414

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Harvey, D. J. 2005. Mass spectrometry. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.5:75.

Author Information

  1. University of Oxford, Oxford, UK

Publication History

  1. Published Online: 15 APR 2005

Abstract

Mass spectrometry is one of the most sensitive and specific techniques for the identification of organic compounds. It has been used for the structural analysis of proteins, glycoproteins, and nucleic acids since the introduction in the late 1980s of the techniques of matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) that have allowed these large biopolymers to be ionized. The article describes these techniques and the types of mass spectrometer that are used to mass-analyze the resulting ions. Methods for obtaining fragment ions from carbohydrates are described, and the use of mass spectrometry for the structural determination of glycoproteins and carbohydrates is briefly discussed.

Keywords:

  • electrospray;
  • fragmentation;
  • mass spectrometry;
  • matrix-assisted laser desorption/ionization (MALDI);
  • carbohydrates