Standard Article

Glycosylphosphatidylinositol anchors – a structural perspective

Part 3. Proteomics

3.5. Proteome Diversity

Basic Techniques and Approaches

  1. Angela Mehlert

Published Online: 15 JAN 2005

DOI: 10.1002/047001153X.g305416

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Mehlert, A. 2005. Glycosylphosphatidylinositol anchors – a structural perspective. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.5:77.

Author Information

  1. University of Dundee, Dundee, UK

Publication History

  1. Published Online: 15 JAN 2005

Abstract

GPI anchors are found attached to many proteins in eukaryotes. The structural characterization of a GPI is challenging owing to the complex nature of the molecule. Certain techniques that take advantage of the unique nature of certain moieties within the GPIs have been used to analyze their structures. Techniques for labeling GPIs with either a radioactive or fluorescent tracer are described, along with a recent “proteomics” style of detecting GPI-anchored proteins from Western blots.

Keywords:

  • glycosylphosphatidylinositol (GPI);
  • C-terminal posttranslational modification;
  • structural characterization;
  • deamination;
  • reduction;
  • methylation linkage analysis;
  • NMR sugar analysis;
  • GC-MS analysis