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Large complexes by X-ray methods

Part 3. Proteomics

3.7. Structural Proteomics

Short Specialist Review

  1. Poul Nissen

Published Online: 15 JUL 2005

DOI: 10.1002/047001153X.g307301

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Nissen, P. 2005. Large complexes by X-ray methods. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.7:99.

Author Information

  1. University of Aarhus, Aarhus, Denmark

Publication History

  1. Published Online: 15 JUL 2005

Abstract

The atomic structures of large complexes such as ribosomes and viruses can be solved by X-ray crystallography. The purification and crystallization, together with data collection and crystallographic phasing, all present unusual problems compared to ordinary high-throughput crystallography. Yet, several examples show how these obstacles can be solved.

Keywords:

  • X ray;
  • crystallography;
  • large assembly;
  • large complex;
  • heavy-atom cluster;
  • virus;
  • ribosome;
  • proteasome;
  • chaperonin;
  • RNA polymerase