Large complexes and molecular machines by electron microscopy
Part 3. Proteomics
3.7. Structural Proteomics
Short Specialist Review
Published Online: 15 APR 2005
Copyright © 2005 John Wiley & Sons, Ltd
Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics
How to Cite
Plitzko, J. M. and Engelhardt, H. 2005. Large complexes and molecular machines by electron microscopy. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics.
- Published Online: 15 APR 2005
Cryo-electron tomography has the unique potential of visualizing the structure of large protein complexes in isolated preparations at high resolution and in their natural context, in unperturbed cells. Tomograms of vitrified cells are essentially three-dimensional images of their entire proteome. They contain the information of the spatial relationship of macromolecules, their interactions, and reveal the assembly of macromolecular machines that are only stable in vivo. Here, we present a brief survey of the principles and developments of cryo-electron microscopy and image reconstruction.
- transmission electron microscopy (TEM);
- electron tomography