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X-ray crystallography

Part 3. Proteomics

3.7. Structural Proteomics

Basic Techniques and Approaches

  1. Susan Firbank

Published Online: 15 JAN 2005

DOI: 10.1002/047001153X.g307403

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Firbank, S. 2005. X-ray crystallography. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.7:104.

Author Information

  1. Wadsworth Centre, New York State Department of Health, Albany, NY, USA

Publication History

  1. Published Online: 15 JAN 2005

Abstract

Since the late 1950s, X-ray crystallography has been used to determine the structure of proteins with known functions. With the dawn of proteomics, protein-structure determination is now being carried out in order to shed light upon proteins of unknown function, with the hope that knowledge of the structure itself will allow, or at least assist in, the identification of possible protein action. This article describes the stages of protein-structure determination by X-ray crystallography, the basic requirements and considerations at each step, and how crystallography can be applied and is being adapted to benefit the structural-genomics community.

Keywords:

  • crystallization;
  • structure determination;
  • model building;
  • phasing;
  • refinement;
  • X-ray diffraction