Electron microscopy as a tool for 3D structure determination in molecular structural biology
Published Online: 15 APR 2005
Copyright © 2005 John Wiley & Sons, Ltd
Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics
How to Cite
Ranson, N. A. 2005. Electron microscopy as a tool for 3D structure determination in molecular structural biology. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics.
- Published Online: 15 APR 2005
Electron microscopy (EM) is a method for determining the structure of large biological assemblies that are difficult targets for X-ray crystallography. The fundamental properties and limitations of image formation in EM are described, along with the basic methods for obtaining and analyzing EM data that are currently in use.
Keywords: structural biology; electron optics; electron microscopy; cryo; 2D crystals; helical symmetry; single particle image processing; 3D reconstruction