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Electron microscopy as a tool for 3D structure determination in molecular structural biology

Part 3. Proteomics

3.7. Structural Proteomics

Basic Techniques and Approaches

  1. Neil A. Ranson

Published Online: 15 APR 2005

DOI: 10.1002/047001153X.g307416

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Ranson, N. A. 2005. Electron microscopy as a tool for 3D structure determination in molecular structural biology. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 3:3.7:106.

Author Information

  1. University of Leeds, Leeds, UK

Publication History

  1. Published Online: 15 APR 2005

Abstract

Electron microscopy (EM) is a method for determining the structure of large biological assemblies that are difficult targets for X-ray crystallography. The fundamental properties and limitations of image formation in EM are described, along with the basic methods for obtaining and analyzing EM data that are currently in use.

Keywords:

  • structural biology;
  • electron optics;
  • electron microscopy;
  • cryo;
  • 2D crystals;
  • helical symmetry;
  • single particle image processing;
  • 3D reconstruction