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Microbial sequence assembly

Part 4. Bioinformatics

4.1. Genome Assembly and Sequencing

Short Specialist Review

  1. George Weinstock

Published Online: 15 JUL 2005

DOI: 10.1002/047001153X.g401304

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Weinstock, G. 2005. Microbial sequence assembly. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 4:4.1:4.

Author Information

  1. Baylor College of Medicine, Houston, TX, USA

Publication History

  1. Published Online: 15 JUL 2005

Abstract

Microbial genomes are assembled by the whole-genome shotgun method, where random sequencing is performed to high redundancy and the sequencing reads are assembled en masse. Although the early microbial genome projects relied on a large insert clone tiling path and a clone-by-clone sequencing approach, current assembly software makes it possible to overcome challenges posed by repeated sequences and perform whole-genome shotgun assemblies.

Keywords:

  • whole-genome shotgun;
  • genome assembly;
  • bioinformatics;
  • draft genome sequence;
  • finished genome sequence