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Errors in sequence assembly and corrections

Part 4. Bioinformatics

4.1. Genome Assembly and Sequencing

Short Specialist Review

  1. Martti T. Tammi1,2,
  2. Björn Andersson1

Published Online: 15 JUL 2005

DOI: 10.1002/047001153X.g401315

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Tammi, M. T. and Andersson, B. 2005. Errors in sequence assembly and corrections. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 4:4.1:7.

Author Information

  1. 1

    Karolinska Institutet, Stockholm, Sweden

  2. 2

    National University of Singapore, Singapore

Publication History

  1. Published Online: 15 JUL 2005

Abstract

The shotgun sequencing method is the most economical and easily automated sequencing technique and has thus become the method of choice. Computer software is used to put the pieces together very much like a jigsaw puzzle, but they can usually only produce a good estimate of a correct assembly, and extensive manual finishing is needed to generate the final sequence.

Keywords:

  • shotgun sequencing;
  • sequencing errors;
  • repeats;
  • polymorphism;
  • coverage