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Genome maps and their use in sequence assembly

Part 4. Bioinformatics

4.1. Genome Assembly and Sequencing

Basic Techniques and Approaches

  1. Paul H. Dear

Published Online: 15 APR 2005

DOI: 10.1002/047001153X.g401411

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Dear, P. H. 2005. Genome maps and their use in sequence assembly. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 4:4.1:8.

Author Information

  1. MRC Laboratory of Molecular Biology, Cambridge, UK

Publication History

  1. Published Online: 15 APR 2005

Abstract

Genome maps give the location of defined sequence features in the genome and serve as a framework around which to assemble the complete genome sequence. Without them, the complete assembly of complex genomes purely from the shotgun sequence data is effectively impossible. Here, I summarize the principle methods that are used to make detailed genome maps and the ways in which mapping and sequencing are integrated to produce finished genome sequences.

Keywords:

  • genome mapping;
  • genome sequencing;
  • sequence assembly;
  • physical mapping;
  • optical mapping;
  • radiation hybrid;
  • HAPPY mapping;
  • shotgun sequencing