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Experimental design

Part 4. Bioinformatics

4.5. Computational Methods for High-throughput Genetic Analysis: Expression Profiling

Specialist Review

  1. Kevin K. Dobbin,
  2. Richard M. Simon

Published Online: 15 NOV 2005

DOI: 10.1002/047001153X.g405205

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics

How to Cite

Dobbin, K. K. and Simon, R. M. 2005. Experimental design. Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics. 4:4.5:52.

Author Information

  1. National Cancer Institute, Bethesda, MD, USA

Publication History

  1. Published Online: 15 NOV 2005

Abstract

Experimental design issues for dual-label and single-label microarray experiments are discussed, including identification of research objectives, avoidance of confounding, allotment of samples to arrays, and dyes in dual-label experiments, dye bias, pooling RNA before labeling, and determination of the number of arrays required to achieve the study objectives.

Keywords:

  • microarrays;
  • experimental design;
  • sample size;
  • dye bias;
  • reference sample