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Dilution Method for Bacterial Density Estimation

  1. Rose E. Gaines Das

Published Online: 15 JUL 2005

DOI: 10.1002/0470011815.b2a06008

Encyclopedia of Biostatistics

Encyclopedia of Biostatistics

How to Cite

Gaines Das, R. E. 2005. Dilution Method for Bacterial Density Estimation. Encyclopedia of Biostatistics. 2.

Author Information

  1. National Institute for Biological Standards, Potters Bar, UK

Publication History

  1. Published Online: 15 JUL 2005

Abstract

There are many methods for the measurement of bacterial mass and concentration. Different measures reflect different characteristics of the bacterial mass, and not all measures reflect the number of viable bacteria. If direct counting and characterization of individual bacterial cells is not possible or feasible, indirect methods must be used. Determination of the “most probable number” and serial dilution assays are indirect methods intended to reflect the number of viable bacteria.

Keywords:

  • colony-forming units;
  • dilution to extinction;
  • most probable number;
  • serial dilution assay;
  • viable bacterial count