Cytoskeletal Networks and Pathways Involved in Endocytosis

  1. Gregory Bock Organizer and
  2. Jamie Goode
  1. David G. Drubin1,
  2. Marko Kaksonen2,
  3. Christopher Toret2 and
  4. Yidi Sun2

Published Online: 7 OCT 2008

DOI: 10.1002/047001766X.ch5

Signalling Networks in Cell Shape and Motility: Novartis Foundation Symposium 269

Signalling Networks in Cell Shape and Motility: Novartis Foundation Symposium 269

How to Cite

Drubin, D. G., Kaksonen, M., Toret, C. and Sun, Y. (2005) Cytoskeletal Networks and Pathways Involved in Endocytosis, in Signalling Networks in Cell Shape and Motility: Novartis Foundation Symposium 269 (eds G. Bock and J. Goode), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/047001766X.ch5

Author Information

  1. 1

    University of California, Berkeley, Department of Molecular & Cell Biology, 16 Barker Hall #3202, Berkeley, CA 94720–3202, USA

  2. 2

    Department of Molecular and Cell Biology, 16 BarkerHall, University of California, Berkeley, CA 94720–3202, USA

Publication History

  1. Published Online: 7 OCT 2008
  2. Published Print: 9 SEP 2005

Book Series:

  1. Novartis Foundation Symposia

Book Series Editors:

  1. Novartis Foundation

ISBN Information

Print ISBN: 9780470011904

Online ISBN: 9780470017661



  • receptor-mediated endocytosis (RME);
  • endocytosis;
  • fluorescence recovery after photobleaching (FRAP);
  • green fluorescent protein (GFP);
  • patch proteins;
  • protein interaction networks;
  • diagrammed interaction network;
  • actin assembly and filament turnover;
  • endocytic internalization process


Until recently, the actin cytoskeleton and the endocytic machinery were thought to operate independently. However, the actin cytoskeleton is an integral part of the cell cortex and there is growing evidence in diverse eukaryotes that F-actin plays a direct role during endocytic internalization. Genetic studies in Saccharomyces cerevisiae have demonstrated that Arp2/3-mediated F-actin assembly is required specifically for the internalization step of endocytosis. Using real-time image analysis, we recently defined a pathway for receptor-mediated endocytosis in budding yeast. Many features of this pathway appear to be conserved widely, indicating that principles derived from our studies in yeast will be directly applicable in more complex eukaryotes. We are pursuing our yeast studies using a combined approach involving image analysis, functional genomics, proteomics and biochemistry. These ongoing studies are providing a broader and deeper understanding of the molecular events of endocytosis, of how forces for actin polymerization are harnessed, and of how steps in the pathway are regulated. Our studies in mammalian cells provide evidence that this pathway is conserved in more complex organisms for endocytic and Golgi tracking events.