Chapter 2. Quantitative TaqMan Real-Time PCR: Diagnostic and Scientific Applications
- Ralph Rapley2,
- Stuart Harbron3
Published Online: 27 SEP 2005
DOI: 10.1002/0470020202.ch2
Copyright © 2004 John Wiley & Sons, Ltd
Book Title
Molecular Analysis and Genome Discovery
Additional Information
How to Cite
Dötsch, J., Schoof, E. and Rascher, W. (2005) Quantitative TaqMan Real-Time PCR: Diagnostic and Scientific Applications, in Molecular Analysis and Genome Discovery (eds R. Rapley and S. Harbron), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/0470020202.ch2
Editor Information
- 2
University of Hertfordshire, UK
- 3
The Enzyme Technology Consultancy, UK
Publication History
- Published Online: 27 SEP 2005
- Published Print: 23 APR 2004
ISBN Information
Print ISBN: 9780471499190
Online ISBN: 9780470020203
- Summary
- Chapter
Keywords:
- TaqMan real-time PCR;
- neuroblastoma;
- haploinsufficiency;
- light cycler system;
- hairpin probes;
- hybridization probes;
- amplifluor primers;
- pseudogenes
Summary
This chapter contains sections titled:
Summary
Introduction
Principles of TaqMan real-time PCR
Reliability and validation of TaqMan real-time PCR
Applications for TaqMan real-time PCR
Limitations and pitfalls in the use of TaqMan real-time PCR
Alternative real-time PCR methods
Future developments
Conclusions
References