• Wiley Online Library will be disrupted on 26 May from 10:00-12:00 BST (05:00-07:00 EDT) for essential maintenance

Chapter 3. Hybridization Probes for Real-Time PCR

  1. Ralph Rapley2,
  2. Stuart Harbron3
  1. Elaine Lyon

Published Online: 27 SEP 2005

DOI: 10.1002/0470020202.ch3

Book Title

Molecular Analysis and Genome Discovery

Molecular Analysis and Genome Discovery

Additional Information

How to Cite

Lyon, E. (2005) Hybridization Probes for Real-Time PCR, in Molecular Analysis and Genome Discovery (eds R. Rapley and S. Harbron), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/0470020202.ch3

Editor Information

  1. 2

    University of Hertfordshire, UK

  2. 3

    The Enzyme Technology Consultancy, UK

Author Information

  1. ARUP Laboratories, Department of Pathology, University of Utah, 500 Chipeta Way, Salt Lake City, UT 84108, USA

Publication History

  1. Published Online: 27 SEP 2005
  2. Published Print: 23 APR 2004

ISBN Information

Print ISBN: 9780471499190

Online ISBN: 9780470020203

SEARCH

CHAPTER TOOLS

Get PDF (118K)

Keywords:

  • pseudogenes;
  • fluorescence resonance energy transfer (FRET);
  • fluorophore;
  • anchor probe;
  • mutation detection;
  • dynamic dot blot;
  • melting curve analysis

Summary

This chapter contains sections titled:

  • Introduction

  • Probe chemistry

  • Fluorescence resonance energy transfer

  • Hybridization probe design

  • FRET applications

  • Summary

  • References

Get PDF (118K)