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Chapter 6. Determination of Nucleic Acid Sequences by Pyrosequencing

  1. Ralph Rapley3,
  2. Stuart Harbron4
  1. Elahe Elahi1,
  2. Mostafa Ronaghi4

Published Online: 27 SEP 2005

DOI: 10.1002/0470020202.ch6

Book Title

Molecular Analysis and Genome Discovery

Molecular Analysis and Genome Discovery

Additional Information

How to Cite

Elahi, E. and Ronaghi, M. (2005) Determination of Nucleic Acid Sequences by Pyrosequencing, in Molecular Analysis and Genome Discovery (eds R. Rapley and S. Harbron), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/0470020202.ch6

Editor Information

  1. 3

    University of Hertfordshire, UK

  2. 4

    The Enzyme Technology Consultancy, UK

Author Information

  1. 1

    Faculty of Science, University of Tehran, Tehran, Iran

  2. 4

    The Enzyme Technology Consultancy, UK

Publication History

  1. Published Online: 27 SEP 2005
  2. Published Print: 23 APR 2004

ISBN Information

Print ISBN: 9780471499190

Online ISBN: 9780470020203

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CHAPTER TOOLS

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Keywords:

  • pyrosequencing;
  • amplicons;
  • pyrograms;
  • expressed sequence tag (EST);
  • streptavidin-coated magnetic beads;
  • single stranded DNA-binding protein (SSB)

Summary

This chapter contains sections titled:

  • Introduction

  • General principles

  • Methodology

  • Applications

  • Future perspectives

  • References

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