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Chapter 8. Overview of Microarrays in Genomic Analysis

  1. Ralph Rapley2,
  2. Stuart Harbron3
  1. Janette K. Burgess

Published Online: 27 SEP 2005

DOI: 10.1002/0470020202.ch8

Book Title

Molecular Analysis and Genome Discovery

Molecular Analysis and Genome Discovery

Additional Information

How to Cite

Burgess, J. K. (2005) Overview of Microarrays in Genomic Analysis, in Molecular Analysis and Genome Discovery (eds R. Rapley and S. Harbron), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/0470020202.ch8

Editor Information

  1. 2

    University of Hertfordshire, UK

  2. 3

    The Enzyme Technology Consultancy, UK

Author Information

  1. Respiratory Research Group, Department of Pharmacology, Bosch Building, D05, University of Sydney, Sydney, NSW, Australia

Publication History

  1. Published Online: 27 SEP 2005
  2. Published Print: 23 APR 2004

ISBN Information

Print ISBN: 9780471499190

Online ISBN: 9780470020203

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CHAPTER TOOLS

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Keywords:

  • biochip;
  • integrated molecular analysis of genomes and their expression (IMAGE);
  • polymerase chain reaction (PCR);
  • oligonucleotide microarray;
  • Affymetrix oligonucleotide arrays;
  • human Gene Expression Index (HuGE Index);
  • European Bioinformatics Institute (EBI);
  • minimum information about a microarray experiment(MIAME)

Summary

This chapter contains sections titled:

  • Introduction

  • What is a microarray?

  • History

  • Production of a cDNA microarray

  • Sources of cDNA

  • Production of an oligonucleotide microarray

  • Array substrates

  • Commercial sources of arrays

  • Isolation of RNA

  • Amplification

  • Target labelling

  • Hybridization

  • Image acquisition

  • Image analysis

  • Data storage

  • Reporting standards

  • Applications to genomic analysis

  • The future?

  • References

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