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Dissimilatory Nitrate Reductase

Part 5. Molybdenum/Tungsten

  1. Maria João Romão,
  2. João Miguel Dias,
  3. Isabel Moura

Published Online: 15 APR 2006

DOI: 10.1002/0470028637.met178

Handbook of Metalloproteins

Handbook of Metalloproteins

How to Cite

João Romão, M., Miguel Dias, J. and Moura, I. 2006. Dissimilatory Nitrate Reductase. Handbook of Metalloproteins. 5.

Author Information

  1. Universidade Nova de Lisboa, Departamento de Química CQFB, Faculdade de Ciências e Tecnologia, Portugal

Publication History

  1. Published Online: 15 APR 2006

Abstract

The dissimilatory nitrate reductase (NAP) from Desulfovibrio desulfuricans ATCC 27774 catalyses the reduction of nitrate to nitrite for respiration. It belongs to the DMSO reductase family of molybdoenzymes and contains one single subunit of 755 amino acids with one molybdenum cofactor and one [4Fe–4S] cluster. The Mo atom is coordinated by two cis-dithiolene groups of two molybdopterin guanine dinucleotides (MGD), by the sulfur atom of Cys140 and by one OH/OH2 ligand. One of the MGD cofactors is directly involved in the electron transfer between the molybdenum center and the [4Fe–4S] cluster.

3D Structure

Keywords:

  • molybdenum enzymes;
  • molybdopterin;
  • nitrate reductase;
  • [4Fe–4S];
  • desulfovibrio desulfuricans