Chapter 3. How to Improve Specificity

  1. Irma Lavagnini1,
  2. Franco Magno1,
  3. Roberta Seraglia2 and
  4. Pietro Traldi2

Published Online: 3 JUL 2006

DOI: 10.1002/0470029021.ch3

Quantitative Applications of Mass Spectrometry

Quantitative Applications of Mass Spectrometry

How to Cite

Lavagnini, I., Magno, F., Seraglia, R. and Traldi, P. (2006) How to Improve Specificity, in Quantitative Applications of Mass Spectrometry, John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/0470029021.ch3

Author Information

  1. 1

    University of Padova, Padova, Italy

  2. 2

    National Council of Research, Padova, Italy

Publication History

  1. Published Online: 3 JUL 2006
  2. Published Print: 7 APR 2006

ISBN Information

Print ISBN: 9780470025161

Online ISBN: 9780470029022



  • chromatographic procedure;
  • capillary zone electrophoresis (CZE);
  • gel permeation chromatography (GPC);
  • supercritical fluid chromatography (SFC);
  • GC/MS and LC/MS;
  • high specificity and selectivity methods;
  • high resolution MID analysis;
  • high resolution mass spectrometry (HRGC/HRMS);
  • MALDI/MS in quantitative polypeptide analysis


This chapter contains sections titled:

  • Choice of a Suitable Chromatographic Procedure

  • Choice of a Suitable Ionization Method

  • An Example of High Specificity and Selectivity Methods: The Dioxin Analysis

  • An Example of MALDI/MS in Quantitative Analysis of Polypeptides: Substance P

  • References