Chapter 1. Basic Light Microscopy
- J. Robin Harris1,
- John Graham2,
- David Rickwood3
Published Online: 3 JUL 2006
DOI: 10.1002/0470033487.ch1
Copyright © 2006 John Wiley & Sons, Ltd
Book Title
Cell Biology Protocols
Additional Information
How to Cite
O'Farrell, M. (2006) Basic Light Microscopy, in Cell Biology Protocols (eds J. R. Harris, J. Graham and D. Rickwood), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/0470033487.ch1
Editor Information
- 1
Institute of Zoology, Johannes Gutenberg-Universität, University of Mainz, D-55099 Mainz, Germany
- 2
JG Research Consultancy, 34 Meadway, Upton Wirral CH49 6IQ, UK
- 3
Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester CO4 3SQ, UK
Publication History
- Published Online: 3 JUL 2006
- Published Print: 27 JAN 2006
ISBN Information
Print ISBN: 9780470847589
Online ISBN: 9780470033487
- Summary
- Chapter
Keywords:
- basic light microscopy;
- numerical aperture (NA);
- bright field microscopy;
- setting Köhler illumination;
- oil immersion objectives;
- phase contrast microscopy;
- phase ring and phase annulus;
- differential interference contrast microscopy;
- dark field microscopy;
- Poly-L-lysine coating
Summary
This chapter contains sections titled:
Introduction
Key components of the compound microscope
Techniques of microscopy
Protocols 1.1 Setting up the microscope for bright field microscopy
Protocols 1.2 Setting Köhler illumination
Protocols 1.3 Focusing procedure
Protocols 1.4 Setting up the microscope for phase contrast microscopy
Protocols 1.5 Setting up the microscope for epifluorescence
Protocols 1.6 Poly-L-lysine coating
References