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Chapter 2. Basic Electron Microscopy

  1. J. Robin Harris3,
  2. John Graham4,
  3. David Rickwood5
  1. J. Robin Harris3,
  2. Jeffrey A. Nickerson1,
  3. Jean Underwood2

Published Online: 3 JUL 2006

DOI: 10.1002/0470033487.ch2

Book Title

Cell Biology Protocols

Cell Biology Protocols

Additional Information

How to Cite

Harris, J. R., Nickerson, J. A. and Underwood, J. (2006) Basic Electron Microscopy, in Cell Biology Protocols (eds J. R. Harris, J. Graham and D. Rickwood), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/0470033487.ch2

Editor Information

  1. 3

    Institute of Zoology, Johannes Gutenberg-Universität, University of Mainz, D-55099 Mainz, Germany

  2. 4

    JG Research Consultancy, 34 Meadway, Upton Wirral CH49 6IQ, UK

  3. 5

    Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester CO4 3SQ, UK

Author Information

  1. 1

    Department of Cell Biology, School of Medicine, University of Massachusetts, 55 Lake Avenue N., Worcester, MA 01655, USA

  2. 2

    Department of Cell Biology, University of Massachusetts Medical School, 55 Lake Avenue, Worcester, MA 01655, USA

  3. 3

    Institute of Zoology, Johannes Gutenberg-Universität, University of Mainz, D-55099 Mainz, Germany

Publication History

  1. Published Online: 3 JUL 2006
  2. Published Print: 27 JAN 2006

ISBN Information

Print ISBN: 9780470847589

Online ISBN: 9780470033487

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CHAPTER TOOLS

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Keywords:

  • Electron microscopy (EM);
  • EM specimen preparation techniques;
  • agarose encapsulation approach;
  • cryoelectron microscopy;
  • immunolabelling of antigens;
  • immunonegative staining;
  • tissue processing and resin embedding;
  • embedded section electron microscopy;
  • Embedment-free-techniques and immunogold antibody staining

Summary

This chapter contains sections titled:

  • Introduction

  • EM methods available

  • Protocols 2.1 Preparation of carbon-formvar, continuous carbon and holey carbon support films

  • Protocols 2.2 The ‘droplet’ negative staining procedure (using continuous carbon, formvar-carbon and holey carbon support films)

  • Protocols 2.3 Immunonegative staining

  • Protocols 2.4 The negative staining-carbon film technique: cell and organelle cleavage

  • Protocols 2.5 Preparation of unstained and negatively stained vitrified specimens

  • Protocols 2.6 Metal shadowing of biological specimens

  • Protocols 2.7 A routine schedule for tissue processing and resin embedding

  • Protocols 2.8 Agarose encapsulation for cell and organelle suspensions

  • Protocols 2.9 Routine staining of thin sections for electron microscopy

  • Protocols 2.10 Post-embedding indirect immunolabelling of thin sections

  • Protocols 2.11 Imaging the nuclear matrix and cytoskeleton by embedment-free electron microscopy

  • References

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