Chapter 3. Cell Culture
- J. Robin Harris2,
- John Graham3,
- David Rickwood4
Published Online: 3 JUL 2006
DOI: 10.1002/0470033487.ch3
Copyright © 2006 John Wiley & Sons, Ltd
Book Title
Cell Biology Protocols
Additional Information
How to Cite
Wilson, A. and Graham, J. (2006) Cell Culture, in Cell Biology Protocols (eds J. R. Harris, J. Graham and D. Rickwood), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/0470033487.ch3
Editor Information
- 2
Institute of Zoology, Johannes Gutenberg-Universität, University of Mainz, D-55099 Mainz, Germany
- 3
JG Research Consultancy, 34 Meadway, Upton Wirral CH49 6IQ, UK
- 4
Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester CO4 3SQ, UK
Publication History
- Published Online: 3 JUL 2006
- Published Print: 27 JAN 2006
ISBN Information
Print ISBN: 9780470847589
Online ISBN: 9780470033487
- Summary
- Chapter
Keywords:
- cell culture;
- tissue disaggregation;
- cold trypsinization;
- collagenase and dispase;
- cryopreservation;
- barrier floatation technique;
- human PBMCs using mixer technique;
- peripheral blood mononuclear cells (PBMCs)
Summary
This chapter contains sections titled:
Cells: isolation and analysis
Mechanical disaggregation of tissue
Protocols 3.1 Tissue disaggregation by mechanical mincing or chopping
Protocols 3.2 Tissue disaggregation by warm trypsinization
Protocols 3.3 Cold trypsinization
Protocols 3.4 Disaggregation using collagenase or dispase
Protocols 3.5 Recovery of cells from effusions
Protocols 3.6 Removal of red blood cells by snap lysis
Protocols 3.7 Removal of red blood cells and dead cells using isopycnic centrifugation
Protocols 3.8 Quantitation of cell counts and viability
Protocols 3.9 Recovery of cells from monolayer cultures
Protocols 3.10 Freezing cells
Protocols 3.11 Thawing cells
Protocols 3.12 Purification of human PBMCs on a density barrier
Protocols 3.13 Purification of human PBMCs using a mixer technique
Protocols 3.14 Purification of human PBMCs using a barrier flotation technique
References