Chapter 8. Peptide Fragmentation Overview

  1. Julia Laskin PhD2 and
  2. Chava Lifshitz PhD3
  1. Vicki H. Wysocki,
  2. Guilong Cheng,
  3. Qingfen Zhang,
  4. Kristin A. Herrmann,
  5. Richard L. Beardsley and
  6. Amy E. Hilderbrand

Published Online: 6 MAR 2006

DOI: 10.1002/047005042X.ch8

Principles of Mass Spectrometry Applied to Biomolecules

Principles of Mass Spectrometry Applied to Biomolecules

How to Cite

Wysocki, V. H., Cheng, G., Zhang, Q., Herrmann, K. A., Beardsley, R. L. and Hilderbrand, A. E. (2006) Peptide Fragmentation Overview, in Principles of Mass Spectrometry Applied to Biomolecules (eds J. Laskin and C. Lifshitz), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/047005042X.ch8

Editor Information

  1. 2

    Fundamental Sciences Division, Pacific Northwest National Laboratory, P.O. Box 999 K8-88, Richland, WA 99352, USA

  2. 3

    Department of Physical Chemistry and The Farkas Center for Light Induced Processes, The Hebrew University of Jerusalem, Jerusalem 91904, Israel

Author Information

  1. Department of Chemistry, Box 210041, University of Arizona, 1306 East University Avenue, Tucson, AZ 85721-0041, USA

Publication History

  1. Published Online: 6 MAR 2006
  2. Published Print: 16 OCT 2006

ISBN Information

Print ISBN: 9780471721840

Online ISBN: 9780470050422



  • accepted peptide fragmentation structures;
  • charge site influence on fragmentation;
  • peptide fragmentation knowledge in algorithm development


This chapter contains sections titled:

  • Introduction

  • Overview of Accepted Peptide Fragment Ion Structures

  • Experimental Influences on Peptide Fragmentation

  • Approaches Used to Study Peptide Fragmentation

  • Influence of Charge Site on Fragmentation

  • Influence of Secondary Structure on Peptide Fragmentation

  • Incorporation of Peptide Fragmentation knowledge into Algorithm Development

  • Remaining Challenges and Future Directions

  • References