Studies on Retinal and Retinal Pigment Epithelial Gene Expression

  1. Gregory Bock Organizer,
  2. Gerry Chader Organizer and
  3. Jamie Goode
  1. Itay Chowers1,†,
  2. Noriko Esumi1,†,
  3. Peter Campochiaro2 and
  4. Donald J. Zack3,*

Published Online: 7 OCT 2008

DOI: 10.1002/0470092645.ch10

Retinal Dystrophies: Functional Genomics to Gene Therapy: Novartis Foundation Symposium 255

Retinal Dystrophies: Functional Genomics to Gene Therapy: Novartis Foundation Symposium 255

How to Cite

Chowers, I., Esumi, N., Campochiaro, P. and Zack, D. J. (2003) Studies on Retinal and Retinal Pigment Epithelial Gene Expression, in Retinal Dystrophies: Functional Genomics to Gene Therapy: Novartis Foundation Symposium 255 (eds G. Bock, G. Chader and J. Goode), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/0470092645.ch10

Author Information

  1. 1

    Guerrieri Center for Genetic Engineering and Molecular Ophthalmology at the Wilmer Eye Institute, The Johns Hopkins University School of Medicine, Baltimore, Maryland, MD 21287, USA

  2. 2

    Guerrieri Center for Genetic Engineering and Molecular Ophthalmology at the Wilmer Eye Institute, the Departments of Neuroscience, The Johns Hopkins University School of Medicine, Baltimore, Maryland, MD 21287, USA

  3. 3

    Guerrieri Center for Genetic Engineering and Molecular Ophthalmology at the Wilmer Eye Institute, the Departments of Neuroscience, and Molecular Biology and Genetics, and McKusick-Nathans Institute of Genetic Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland, MD 21287, USA

  1. These authors have contributed equally to the chapter.

*Ophthalmology, Molecular Biology and Genetics, Johns Hopkins University School of Medicine, 809 Maumenee, 600 N. Wolfe Street, Baltimore, MD 21287, USA

Publication History

  1. Published Online: 7 OCT 2008
  2. Published Print: 9 DEC 2003

Book Series:

  1. Novartis Foundation Symposia

Book Series Editors:

  1. Novartis Foundation

ISBN Information

Print ISBN: 9780470853573

Online ISBN: 9780470092644

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Summary

The ‘completion’ of the murine and human genomes and creation of high-density expressed sequence tag (EST) databases from multiple tissues and multiple species, coupled with the development of high-throughput expression profiling approaches such as microarrays and Serial Analysis of Gene Expression (SAGE), is making possible the in-depth analysis of gene expression patterns in health and disease to an extent that was not previously possible. Such new information is providing insight into normal function, and into how normal function is altered in disease. Efforts have begun, and are accelerating, in the application of expression profiling to the study of the retina and retinal pigment epithelium (RPE). In this chapter we will review progress in this area. We will also discuss technical issues that make expression studies of the RPE particularly challenging, and share our experience in methodological approaches to overcome these challenges.