Chapter 8. Mammalian Artificial Chromosomes: Prospects for Gene Therapy
- Anthony Meager
Published Online: 10 DEC 2001
DOI: 10.1002/0470842385.ch8
Copyright © 1999 John Wiley & Sons, Ltd
Book Title
Gene Therapy Technologies, Applications and Regulations: From Laboratory to Clinic
Additional Information
How to Cite
Ebersole, T. A. and Farr, C. J. (2001) Mammalian Artificial Chromosomes: Prospects for Gene Therapy, in Gene Therapy Technologies, Applications and Regulations: From Laboratory to Clinic (ed A. Meager), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/0470842385.ch8
Editor Information
Division of Immunobiology, The National Institute for Biological Standards and Control, South Mimms, UK
Publication History
- Published Online: 10 DEC 2001
- Published Print: 17 SEP 1999
ISBN Information
Print ISBN: 9780471967095
Online ISBN: 9780470842386
- Summary
- Chapter
Keywords:
- human chromosome;
- centromere;
- origin of replication;
- telomere;
- yeast artificial chromosome;
- capacity;
- intron;
- regulatory element
Summary
A mammalian artificial chromosome would mimic the natural means of transferring genetic information from one cell to another and could be used to carry larger gene complexes than current vector systems can handle, possibly tens of millions of base pairs. A centromere, origins of replication and two telomeres are sufficient to generate a yeast artificial chromosome but it is not known whether mammalian chromosomes require extra features. For gene therapy applications, they would need to be invisible to the immune system and easy to deliver to target cells. Attempts to build such artificial chromosomes are proceeding from both directions: deleting elements of existing human chromosomes and assembling the individual components, although for mammalian chromosomes these have not all been defined molecularly.