Chapter 5. Manipulating Biomolecules

  1. C. U. M. Smith

Published Online: 11 FEB 2003

DOI: 10.1002/0470857498.ch5

Elements of Molecular Neurobiology, Third Edition

Elements of Molecular Neurobiology, Third Edition

How to Cite

Smith, C. U. M. (2002) Manipulating Biomolecules, in Elements of Molecular Neurobiology, Third Edition, John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/0470857498.ch5

Author Information

  1. Department of Vision Sciences, Aston University, Birmingham, UK

Publication History

  1. Published Online: 11 FEB 2003
  2. Published Print: 15 SEP 2002

ISBN Information

Print ISBN: 9780470843536

Online ISBN: 9780470857496

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Summary

This chapter contains sections titled:

  • Restriction Endonucleases

  • Separation of Restriction Fragments

  • Restriction Maps

  • Recombination

  • Cloning

  • Isolating Bacteria Containing Recombinant Plasmids or Phage

  • The ‘Shotgun’ Construction of ‘Genomic’ Gene Libraries

  • A Technique for Finding a Gene in the Library

  • Construction of a ‘cDNA’ Gene Library

  • Fishing for Genes in a cDNA Library

  • Positional Cloning

  • The Polymerase Chain Reaction (PCR)

  • Sequence Analysis of DNA

  • Prokaryotic Expression Vectors for Eukaryotic DNA

  • Xenopus Oocyte as an Expression Vector for Membrane Proteins

  • Site-directed Mutagenesis

  • Gene Targeting and Knockout Genetics

  • Targeted Gene Expression

  • Hybridisation Histochemistry

  • DNA Chips

  • Conclusion