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Chapter 10. SNP Discovery and PCR-Based Assay Design: From In Silico Data to the Laboratory Experiment

  1. Michael R. Barnes4,
  2. Ian C. Gray5
  1. Ellen Vieux1,
  2. Gabor Marth2,
  3. Pui-Yan Kwok3

Published Online: 22 MAY 2003

DOI: 10.1002/0470867302.ch10

Book Title

Bioinformatics for Geneticists

Bioinformatics for Geneticists

Additional Information

How to Cite

Vieux, E., Marth, G. and Kwok, P.-Y. (2003) SNP Discovery and PCR-Based Assay Design: From In Silico Data to the Laboratory Experiment, in Bioinformatics for Geneticists (eds M. R. Barnes and I. C. Gray), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/0470867302.ch10

Editor Information

  1. 4

    Genetic Bioinformatics, GlaxoSmithKline Pharmaceuticals, New Frontiers Science Park (North), Third Avenue, Harlow, Essex CM19 5AW, UK

  2. 5

    Discovery Genetics, GlaxoSmithKline Pharmaceuticals, New Frontiers Science Park (North), Third Avenue, Harlow, Essex CM19 5AW, UK

Author Information

  1. 1

    Washington University School of Medicine, 660 S. Euclid Ave, Box 8123, St Louis, MO 63110, USA

  2. 2

    National Center for Biotechnology Information, NLM, NIH, 8600 Rockville Pike, Building 45, Room 5AS29, Bethesda, Maryland 20894, USA

  3. 3

    University of California, San Francisco, 505 Parnassus Ave, Long 1332A, Box 0130, San Francisco, CA 94143-0130, USA

Publication History

  1. Published Online: 22 MAY 2003
  2. Published Print: 21 FEB 2003

ISBN Information

Print ISBN: 9780470843932

Online ISBN: 9780470867303

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CHAPTER TOOLS

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Summary

This chapter contains sections titled:

  • Introduction

  • SNP Identification

  • PCR Primer Design

  • Broader PCR Assay Design Issues

  • Primer Selection

  • Problems Related to SNP Assay Validation

  • Conclusion

  • References

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