Chapter 11. The In-situ Polymerase Chain Reaction
- John Crocker2,
- Paul G. Murray3
Published Online: 15 AUG 2003
DOI: 10.1002/0470867949.ch11
Copyright © 2003 John Wiley & Sons, Ltd
Book Title
Molecular Biology in Cellular Pathology
Additional Information
How to Cite
O'Leary, J. J., Martin, C. and Sheils, O. (2003) The In-situ Polymerase Chain Reaction, in Molecular Biology in Cellular Pathology (eds J. Crocker and P. G. Murray), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/0470867949.ch11
Editor Information
- 2
Department of Cellular Pathology, Birmingham Heartlands Hospital, Bordesley Green East, Birmingham B9 5SS, UK
- 3
Department of Pathology, The Medical School, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
Publication History
- Published Online: 15 AUG 2003
- Published Print: 22 APR 2003
ISBN Information
Print ISBN: 9780470844755
Online ISBN: 9780470867945
- Summary
- Chapter
Summary
This chapter contains sections titled:
Introduction
Overview of the Methodology
In-cell PCR Technologies
In-cell Amplification of DNA
Detection of Amplicons
Reaction, Tissue and Detection Controls for Use with In-cell DNA PCR Assays
In-cell RNA Amplification
Problems Encountered with In-cell PCR Amplification
Amplicon Diffusion and Back Diffusion
Future Work with In-cell PCR-based Assays
References