Chapter 12. TaqMan® Technology and Real-Time Polymerase Chain Reaction
- John Crocker2,
- Paul G. Murray3
Published Online: 15 AUG 2003
DOI: 10.1002/0470867949.ch12
Copyright © 2003 John Wiley & Sons, Ltd
Book Title
Molecular Biology in Cellular Pathology
Additional Information
How to Cite
O'Leary, J. J., Sheils, O., Martin, C. and Crowley, A. (2003) TaqMan® Technology and Real-Time Polymerase Chain Reaction, in Molecular Biology in Cellular Pathology (eds J. Crocker and P. G. Murray), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/0470867949.ch12
Editor Information
- 2
Department of Cellular Pathology, Birmingham Heartlands Hospital, Bordesley Green East, Birmingham B9 5SS, UK
- 3
Department of Pathology, The Medical School, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
Publication History
- Published Online: 15 AUG 2003
- Published Print: 22 APR 2003
ISBN Information
Print ISBN: 9780470844755
Online ISBN: 9780470867945
- Summary
- Chapter
Summary
This chapter contains sections titled:
Introduction
Probe Technologies
TaqMan® Probe and Chemistry (First Generation)
Second Generation TaqMan® Probes
Hybridisation
TaqMan® PCR Conditions
Standards for Quantitative PCR
Interpretation of Results
End-point Detection
Real-time Detection
Relative Quantitation
Reference Genes
Specific TaqMan® PCR Applications
References