Developmental Transitions in Cardiac Conduction

  1. Derek J. Chadwick Organizer and
  2. Jamie Goode
  1. Michiko Watanabe1,
  2. Emil Thomas Chuck2,
  3. Florence Rothenberg3 and
  4. David S. Rosenbaum3

Published Online: 7 OCT 2008

DOI: 10.1002/0470868066.ch5

Development of the Cardiac Conduction System: Novartis Foundation Symposium 250

Development of the Cardiac Conduction System: Novartis Foundation Symposium 250

How to Cite

Watanabe, M., Chuck, E. T., Rothenberg, F. and Rosenbaum, D. S. (2003) Developmental Transitions in Cardiac Conduction, in Development of the Cardiac Conduction System: Novartis Foundation Symposium 250 (eds D. J. Chadwick and J. Goode), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/0470868066.ch5

Author Information

  1. 1

    Division of Pediatric Cardiology, Department of Pediatrics, Rainbow Babies and Children's Hospital, University Hospitals of Cleveland/Case Western Reserve University School of Medicine, 11100 Euclid Avenue, Cleveland, OH 44106, USA

  2. 2

    Neonatal Perinatal Research Institute, Division of Neonatology, Department of Pediatrics, Box 3179, Duke University Medical Center, Durham, NC 27710, USA

  3. 3

    Heart and Vascular Research Center, MetroHealth Campus, Case Western Reserve University, Cleveland, OH, USA

Publication History

  1. Published Online: 7 OCT 2008
  2. Published Print: 20 JUN 2003

Book Series:

  1. Novartis Foundation Symposia

Book Series Editors:

  1. Novartis Foundation

ISBN Information

Print ISBN: 9780470850350

Online ISBN: 9780470868065



The proper sequence of electrical activation of the mature four-chambered heart requires specialized conduction pathways including the His–Purkinje system and a nearly complete separation of the atrial and ventricular myocardium. We tracked the emergence of the structure of the mature His–Purkinje system in the developing chicken embryo with anti-polysialylated neural cell adhesion molecule (PSA-NCAM) and the HNK1 antibody against a sulfated carbohydrate epitope. The function of the His–Purkinje system was assayed using extracellular electrodes and high-resolution voltage-sensitive two-dimensional optical mapping. The appearance of the mature form of the His–Purkinje system delineated by the markers coincided with the onset of the mature electrophysiological pattern of ventricular activation. These data suggest that, at the completion of ventricular septation, the His–Purkinje system undergoes critical structural and functional transitions that impact on the global pattern of conduction and contraction of the developing four-chambered heart.