His–Purkinje Lineages and Development

  1. Derek J. Chadwick Organizer and
  2. Jamie Goode
  1. Robert G. Gourdie1,
  2. Brett S. Harris1,2,
  3. Jacqueline Bond1,
  4. Angela M. Edmondson2,
  5. Gang Cheng1,
  6. David Sedmera1,
  7. Terrence X. O'Brien1,2,
  8. Takashi Mikawa3 and
  9. Robert P. Thompson1

Published Online: 7 OCT 2008

DOI: 10.1002/0470868066.ch7

Development of the Cardiac Conduction System: Novartis Foundation Symposium 250

Development of the Cardiac Conduction System: Novartis Foundation Symposium 250

How to Cite

Gourdie, R. G., Harris, B. S., Bond, J., Edmondson, A. M., Cheng, G., Sedmera, D., O'Brien, T. X., Mikawa, T. and Thompson, R. P. (2003) His–Purkinje Lineages and Development, in Development of the Cardiac Conduction System: Novartis Foundation Symposium 250 (eds D. J. Chadwick and J. Goode), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/0470868066.ch7

Author Information

  1. 1

    Department of Cell Biology and Anatomy, Cardiovascular Developmental Biology Center, Medical University of South Carolina, 171 Ashley Avenue, Charleston, SC 29425, USA

  2. 2

    Ralph H. Johnson Department of Veteran Affairs Medical Center, Charleston, USA

  3. 3

    Department of Cell Biology, Cornell University Medical College, 1300 York Avenue, New York, NY 10021, USA

Publication History

  1. Published Online: 7 OCT 2008
  2. Published Print: 20 JUN 2003

Book Series:

  1. Novartis Foundation Symposia

Book Series Editors:

  1. Novartis Foundation

ISBN Information

Print ISBN: 9780470850350

Online ISBN: 9780470868065



The heartbeat is initiated and coordinated by a multi-component set of specialized muscle tissues collectively referred to as the pacemaking and conduction system. Over the last few years, impetus has gathered into unravelling the cellular and molecular processes that regulate differentiation and integration of this essential cardiac network. One focus of our collective work has been the developmental history of cells comprising His–Purkinje tissues of the conduction system. This interest in part arose from studies of the expression of connexins in periarterial Purkinje fibres of the chick heart. Using lineage-tracing strategies, including those based on replication-defective retroviruses and adenoviruses, it has been shown that conduction cells are derived from multipotent, cardiomyogenic progenitors in the tubular heart. Moreover, heterogeneity within myocardial clones has indicated that the elaboration of the conduction system in the chick embryo occurs by progressive, localized recruitment from within this pool of cardiomyogenic cells. Cell birth dating has revealed that inductive conscription of cells to central elements of the conduction system (e.g. the His bundle) precedes recruitment to the peripheral components of the network (i.e. subendocardial and periarterial Purkinje fibres). Birth dating studies in rodents suggest an analogous recruitment process is occurring in this species. In addition to summarizing earlier work, this chapter provides information on ongoing studies of cell–cell signalling and transcriptional mechanisms that may regulate the development of His–Purkinje tissues.