Induction and Patterning of the Purkinje Fibre Network

  1. Derek J. Chadwick Organizer and
  2. Jamie Goode
  1. Takashi Mikawa1,
  2. Robert G. Gourdie2,
  3. Kimiko Takebayashi-Suzuki1,
  4. Nobuyuki Kanzawa1,
  5. Jeanette Hyer1,
  6. David J. Pennisi1,
  7. Clifton P. Poma1,
  8. Maxim Shulimovich1,
  9. Kevin G. Diaz1,
  10. John Layliev1 and
  11. Aparna Prasad1

Published Online: 7 OCT 2008

DOI: 10.1002/0470868066.ch9

Development of the Cardiac Conduction System: Novartis Foundation Symposium 250

Development of the Cardiac Conduction System: Novartis Foundation Symposium 250

How to Cite

Mikawa, T., Gourdie, R. G., Takebayashi-Suzuki, K., Kanzawa, N., Hyer, J., Pennisi, D. J., Poma, C. P., Shulimovich, M., Diaz, K. G., Layliev, J. and Prasad, A. (2003) Induction and Patterning of the Purkinje Fibre Network, in Development of the Cardiac Conduction System: Novartis Foundation Symposium 250 (eds D. J. Chadwick and J. Goode), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/0470868066.ch9

Author Information

  1. 1

    Department of Cell Biology, Cornell University Medical College, 1300 York Avenue, New York, NY 10021, USA

  2. 2

    Department of Cell Biology and Anatomy, Medical University of South Carolina, 171 Ashley Avenue, Charleston, SC 29425, USA

Publication History

  1. Published Online: 7 OCT 2008
  2. Published Print: 20 JUN 2003

Book Series:

  1. Novartis Foundation Symposia

Book Series Editors:

  1. Novartis Foundation

ISBN Information

Print ISBN: 9780470850350

Online ISBN: 9780470868065



Impulse-conducting Purkinje cells differentiate from myocytes during embryogenesis. In the embryonic chicken heart, this conversion of contractile myocytes into conduction cells occurs subendocardially and periarterially. The unique sites of Purkinje fibre differentiation suggest that a shear stress-induced paracrine signal from the endocardium and arterial beds may induce adjacent myocytes to differentiate into conduction cells. Consistent with this model, Purkinje fibre marker genes can be induced in cultured embryonic myocytes by endothelin (ET), an endothelial cell-derived signalling peptide. This inductive response is, however, gradually lost from myocytes as embryos develop, and mature myocytes express only genes characteristic of hypertrophy in response to ET. In vivo, active ET is produced, through proteolytic processing, from its precursor by ET-converting enzyme 1 (ECE1) and triggers signalling by binding to its receptors, ETA and ETB. In the embryonic heart, the expression of these ET signalling components changes dynamically, defining the site and timing of Purkinje fibre differentiation within the ventricular myocardium during chick embryogenesis.