Unit

UNIT 1.5 The Use of Fish-Derived Cell Lines for Investigation of Environmental Contaminants

  1. Vivian R. Dayeh1,
  2. Niels C. Bols1,
  3. Kristin Schirmer2,
  4. Lucy E.J. Lee3

Published Online: 1 MAY 2003

DOI: 10.1002/0471140856.tx0105s15

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Dayeh, V. R., Bols, N. C., Schirmer, K. and Lee, L. E. 2003. The Use of Fish-Derived Cell Lines for Investigation of Environmental Contaminants. Current Protocols in Toxicology. 15:1.5:1.5.1–1.5.17.

Author Information

  1. 1

    University of Waterloo, Waterloo, Canada

  2. 2

    UFZ-Centre for Environmental Research, Leipzig, Germany

  3. 3

    Wilfrid Laurier University, Waterloo, Canada

Publication History

  1. Published Online: 1 MAY 2003
  2. Published Print: FEB 2003

This is not the most recent version of the article. View current version (1 MAY 2013)

Abstract

This unit describes protocols for growing salmonid cell lines and using them in in vitro toxicology studies. Cell viability of cultures is assessed with three indicator dyes: alamar blue for metabolic activity, CFDA-AM for membrane integrity, and neutral red for lysosomal activity. These protocols are essential tools for investigating environmental toxicity at the cellular level.