Unit

UNIT 2.2 Determination of Apoptosis and Necrosis

  1. Boris Zhivotovsky,
  2. Afshin Samali,
  3. Sten Orrenius

Published Online: 1 MAY 2001

DOI: 10.1002/0471140856.tx0202s00

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Zhivotovsky, B., Samali, A. and Orrenius, S. 2001. Determination of Apoptosis and Necrosis. Current Protocols in Toxicology. 00:2.2:2.2.1–2.2.34.

Author Information

  1. Karolinska Institute, Stockholm, Sweden

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAY 1999

Abstract

Cell death occurs by one of two mechanisms: apoptosis (programmed cell death) or necrosis due to cellular injury or trauma. Each method has distinct morphological and biochemical characteristics. Distinguishing between apoptosis and necrosis depends selection of appropriate assays and proper timing. This unit provides an extensive series of assays that measure membrane integrity, cell size and DNA content, state of chromatin, DNA fragmentation, and caspase activation. The results of these assays allow one to distinguish between apoptosis and necrosis.