UNIT 2.4 Measurement of Lipid Peroxidation

  1. Christopher A. Reilly,
  2. Steven D. Aust

Published Online: 1 MAY 2001

DOI: 10.1002/0471140856.tx0204s00

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Reilly, C. A. and Aust, S. D. 2001. Measurement of Lipid Peroxidation. Current Protocols in Toxicology. 00:2.4:2.4.1–2.4.13.

Author Information

  1. Utah State University, Logan, Utah

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAY 1999


There is currently considerable interest in what is termed “oxidative stress,” or the oxidation of biological macromolecules, with emphasis on its involvement in various diseases and toxicities and methods to limit either its occurrence or effects. This unit describes traditional methods to measure the extent or rate of lipid peroxidations, including assays for conjugated dienes, lipid hydroperoxides, the polyunsaturated lipid breakdown product malondialdehyde, and hemolysis, along with discussion of alternative methods.