Unit

UNIT 2.5 Measurements of Intracellular Free Calcium Concentration in Biological Systems

  1. Karlene K. Gunter,
  2. Thomas E. Gunter

Published Online: 1 MAY 2001

DOI: 10.1002/0471140856.tx0205s01

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Gunter, K. K. and Gunter, T. E. 2001. Measurements of Intracellular Free Calcium Concentration in Biological Systems. Current Protocols in Toxicology. 1:2.5:2.5.1–2.5.52.

Author Information

  1. University of Rochester, Rochester, New York

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: AUG 1999

Abstract

Intracellular calcium plays a role in signaling and as a second messenger in many types of cells and its concentration is closely regulated in cells. Two techniques for measuring intracellular free calcium are using fluorescent ratiometric and nonratiometric probes are described in this unit: fluorescent spectroscopy to measure calcium concentrations in a suspension of cells and fluorescent digital imaging microscopy (FDIM) to measure calcium concentrations in plated cells.