UNIT 2.5 Measurements of Intracellular Free Calcium Concentration in Biological Systems
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Gunter, K. K. and Gunter, T. E. 2001. Measurements of Intracellular Free Calcium Concentration in Biological Systems. Current Protocols in Toxicology. 1:2.5:2.5.1–2.5.52.
- Published Online: 1 MAY 2001
- Published Print: AUG 1999
Intracellular calcium plays a role in signaling and as a second messenger in many types of cells and its concentration is closely regulated in cells. Two techniques for measuring intracellular free calcium are using fluorescent ratiometric and nonratiometric probes are described in this unit: fluorescent spectroscopy to measure calcium concentrations in a suspension of cells and fluorescent digital imaging microscopy (FDIM) to measure calcium concentrations in plated cells.