UNIT 2.6 In Vitro Methods for Detecting Cytotoxicity
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Ehrich, M. and Sharova, L. 2001. In Vitro Methods for Detecting Cytotoxicity. Current Protocols in Toxicology. 3:2.6.1–2.6.27.
- Published Online: 1 MAY 2001
- Published Print: FEB 2000
Toxicant-induced damage to cells (cytotoxicity) can depress cell growth, compromise intracellular metabolic processes, and/or cause loss of cell viability. Methods that indicate these cytotoxic changes following toxicant exposures are provided, including [3H]thymidine uptake to measure cell growth, MTT dye conversion to detect changes in metabolic activity, and trypan blue uptake to indicate loss of cell viability.