UNIT 2.9 Measurement of Expression of the HSP70 Protein Family
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Bargeron, M. and Sharp, F. R. 2001. Measurement of Expression of the HSP70 Protein Family. Current Protocols in Toxicology. 7:2.9:2.9.1–2.9.40.
- Published Online: 1 MAY 2001
- Published Print: FEB 2001
The heat shock protein family of proteins include a number of proteins of similar sizes that are inducible by a wide variety of “stress” stimuli including heat shock, ischemia, toxic metals, amino acid analogs, and some chemotherapeutic and anesthetic chemicals. The functions of these proteins are diverse but are generally associated with molecular chaperoning of proteins. This unit contains a wide variety of protocols for analyzing expression of this family at the transcriptional and translational level: northern blot hybridization, in situ hybridization, metabolic labeling and SDS-PAGE, and immunoblotting.