Unit

UNIT 2.9 Measurement of Expression of the HSP70 Protein Family

  1. Marcelle Bargeron1,
  2. Frank R. Sharp2

Published Online: 1 MAY 2001

DOI: 10.1002/0471140856.tx0209s07

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Bargeron, M. and Sharp, F. R. 2001. Measurement of Expression of the HSP70 Protein Family. Current Protocols in Toxicology. 7:2.9:2.9.1–2.9.40.

Author Information

  1. 1

    Lilly Research Laboratories, Indianapolis, Indiana

  2. 2

    University of Cincinnati Medical Center, Cincinnati, Ohio

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 2001

Abstract

The heat shock protein family of proteins include a number of proteins of similar sizes that are inducible by a wide variety of “stress” stimuli including heat shock, ischemia, toxic metals, amino acid analogs, and some chemotherapeutic and anesthetic chemicals. The functions of these proteins are diverse but are generally associated with molecular chaperoning of proteins. This unit contains a wide variety of protocols for analyzing expression of this family at the transcriptional and translational level: northern blot hybridization, in situ hybridization, metabolic labeling and SDS-PAGE, and immunoblotting.