Unit

UNIT 2.16 Fluorescent pH and Oxygen Probes of the Assessment of Mitochondrial Toxicity in Isolated Mitochondria and Whole Cells

  1. James Hynes1,
  2. Edward Natoli Jr.2,
  3. Yvonne Will2

Published Online: 1 MAY 2009

DOI: 10.1002/0471140856.tx0216s40

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Hynes, J., Natoli, E. and Will, Y. 2009. Fluorescent pH and Oxygen Probes of the Assessment of Mitochondrial Toxicity in Isolated Mitochondria and Whole Cells. Current Protocols in Toxicology. 40:2.16:2.16.1–2.16.22.

Author Information

  1. 1

    Luxcel Biosciences, BioInnovation Centre, UCC, Cork, Ireland

  2. 2

    Pfizer Global Research, Groton, Connecticut

Publication History

  1. Published Online: 1 MAY 2009
  2. Published Print: MAY 2009

Abstract

Oxygen consumption measurements provide information on the activity of the mitochondrial electron transport chain and such measurements can be carried out on both isolated mitochondria and whole cells. Additional information is available on glycolytic metabolism by assessing extracellular acidification. The protocols in this unit describe how recently developed fluorescent pH- and O2-sensitive probes can be used as tools for the assessment of mitochondrial toxicity and metabolic function in standard microtiter plates. In addition, protocols for the isolation of mitochondria from different tissue sources are provided. Curr. Protoc. Toxicol. 40:2.16.1-2.16.22. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • mitochondria;
  • toxicity;
  • oxygen consumption;
  • extracellular acidification