UNIT 2.21 Measuring Intracellular hsp70 in Leukocytes by Flow Cytometry

  1. Linda L. Agnew

Published Online: 1 AUG 2011

DOI: 10.1002/0471140856.tx0221s49

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Agnew, L. L. 2011. Measuring Intracellular hsp70 in Leukocytes by Flow Cytometry. Current Protocols in Toxicology. 49:2.21:2.21.1–2.21.12.

Author Information

  1. Centre for Bioactive Discovery in Health and Ageing, School of Science and Technology, University of New England, Armidale, Australia

Publication History

  1. Published Online: 1 AUG 2011
  2. Published Print: AUG 2011


Heat shock or stress proteins are constitutively expressed redox-sensitive proteins, the synthesis of which is induced in almost all organisms exposed to a range of stressors, including heat shock, oxidative stress, free radicals, UV radiation, and heavy metals. This unit details a method, with supporting protocols, for the measurement of their expression in peripheral blood leukocytes by flow cytometry. Curr. Protoc. Toxicol. 49:2.21.1-2.21.12. © 2011 by John Wiley & Sons, Inc.


  • heat shock;
  • stress proteins;
  • flow cytometry;
  • leukocyte