UNIT 2.21 Measuring Intracellular hsp70 in Leukocytes by Flow Cytometry
Published Online: 1 AUG 2011
Copyright © 2011 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Agnew, L. L. 2011. Measuring Intracellular hsp70 in Leukocytes by Flow Cytometry. Current Protocols in Toxicology. 49:2.21:2.21.1–2.21.12.
- Published Online: 1 AUG 2011
- Published Print: AUG 2011
Heat shock or stress proteins are constitutively expressed redox-sensitive proteins, the synthesis of which is induced in almost all organisms exposed to a range of stressors, including heat shock, oxidative stress, free radicals, UV radiation, and heavy metals. This unit details a method, with supporting protocols, for the measurement of their expression in peripheral blood leukocytes by flow cytometry. Curr. Protoc. Toxicol. 49:2.21.1-2.21.12. © 2011 by John Wiley & Sons, Inc.
- heat shock;
- stress proteins;
- flow cytometry;