Unit

UNIT 3.8 Methods for Measuring DNA Adducts and Abasic Sites I: Isolation, Purification, and Analysis of DNA Adducts in Intact DNA

  1. James A. Swenberg,
  2. Amy-Joan L. Ham,
  3. Kevin S. McDorman,
  4. Eric J. Morinello,
  5. Jun Nakamura,
  6. Robert Schoonhoven

Published Online: 1 AUG 2002

DOI: 10.1002/0471140856.tx0308s12

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Swenberg, J. A., Ham, A.-J. L., McDorman, K. S., Morinello, E. J., Nakamura, J. and Schoonhoven, R. 2002. Methods for Measuring DNA Adducts and Abasic Sites I: Isolation, Purification, and Analysis of DNA Adducts in Intact DNA. Current Protocols in Toxicology. 12:3.8:3.8.1–3.8.18.

Author Information

  1. University of North Carolina, Chapel Hill, North Carolina

Publication History

  1. Published Online: 1 AUG 2002
  2. Published Print: MAY 2002

Abstract

The major event involved in the formation of mutations and the initiation and progression of cancer is the induction of DNA damage by reactive intermediates arising from exposure to endogenous and exogenous chemicals. Many electrophilic metabolites of chemicals covalently bind to the bases of DNA causing specific DNA adducts. This unit includes protocols for preparing samples of intact DNA and adduct analysis to quantify the number of adducts that can potentially cause mutagenic or carcinogenic damage.