UNIT 3.9 Methods for Measuring DNA Adducts and Abasic Sites II: Methods for Measurement of DNA Adducts
Published Online: 1 AUG 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Swenberg, J. A., Ham, A.-J. L., Koc, H., La, D. K., Morinello, E. J., Pachkowski, B. F., Ranasinghe, A. and Upton, P. B. 2002. Methods for Measuring DNA Adducts and Abasic Sites II: Methods for Measurement of DNA Adducts. Current Protocols in Toxicology. 12:3.9:3.9.1–3.9.35.
- Published Online: 1 AUG 2002
- Published Print: MAY 2002
This unit contains protocols for analyzing DNA adducts separated from the DNA backbone. HPLC is used to quantify total guanine or ribo- or deoxynucleotides as well as methods for analyzing specific adducts. These methods include HPLC with electrochemical detection, immunoaffininty chromatography to enrich for specific adducts, and gas and liquid chromatography in combination with HPLC and mass spectrometry.