Unit

UNIT 4.11 Techniques for Measuring the Activity of Carboxylic Acid:CoA Ligase and Acyl-CoA:Amino Acid N-Acyltransferase: The Amino Acid Conjugation Pathway

  1. Michael Kelley,
  2. Donald A. Vessey

Published Online: 1 FEB 2003

DOI: 10.1002/0471140856.tx0411s14

Current Protocols in Toxicology

Current Protocols in Toxicology

How to Cite

Kelley, M. and Vessey, D. A. 2003. Techniques for Measuring the Activity of Carboxylic Acid:CoA Ligase and Acyl-CoA:Amino Acid N-Acyltransferase: The Amino Acid Conjugation Pathway. Current Protocols in Toxicology. 14:4.11:4.11.1–4.11.16.

Author Information

  1. Veterans Administration Medical Center, San Francisco, California

Publication History

  1. Published Online: 1 FEB 2003
  2. Published Print: NOV 2002

Abstract

A wide variety of xenobiotic carboxylic acids are metabolized to their amino acid conjugates via a pathway that exists primarily in liver and kidney. This conjugation occurs in a two-step pathway catalyzed by two distince types of enzymes, ligases and transferases. Measurements of acyl-CoA ligase activity include monitoring the rate of appearance of AMP or PPi, or the CoA adduct. N-acyltransferases catalyze formation of an amino acid conjugate from the CoA-activated intermediate, releasing CoA. This reaction is monitored by following the release of free CoA or the disappearance of the acyl-CoA adduct.