UNIT 4.14 Biotransformation Studies Using Rat Proximal Tubule Cells
Published Online: 1 SEP 2004
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Schaaf, G. J., Maas, R. F. M. and Fink-Gremmels, J. 2004. Biotransformation Studies Using Rat Proximal Tubule Cells. Current Protocols in Toxicology. 21:4.14:4.14.1–4.14.16.
- Published Online: 1 SEP 2004
- Published Print: AUG 2004
The proximal tubule is the main target for nephrotoxic substances due to its specific properties, including efficient drug transport and biotransformation potential. The availability of a pure population of proximal tubule cells (PT cells) as a model to study a range of biological, pharmacological, and toxicological parameters is, therefore, of great value. A two-step PT cell–isolation procedure, based on density-gradient centrifugation, is described; this procedure can easily be introduced into each laboratory setting. The procedure routinely yields a highly pure PT cell population, comprising 20–40 × 106 cells, which can be used for preparation of subcellular fractions or brought into primary culture.
- Proximal tubule cell isolation;
- primary cultures