UNIT 4.16 Evaluation of the Cytochrome b5/Cytochrome b5 Reductase Pathway
Published Online: 1 JUN 2005
Copyright © 2005 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Trepanier, L. A., Bajad, S. U. and Kurian, J. R. 2005. Evaluation of the Cytochrome b5/Cytochrome b5 Reductase Pathway. Current Protocols in Toxicology. 24:4.16:4.16.1–4.16.17.
- Published Online: 1 JUN 2005
- Published Print: MAY 2005
NADH cytochrome b5 reductase (b5R; EC 184.108.40.206; Diaphorase I; NADH: ferricytochrome b5 oxidoreductase) is an FAD-containing protein, which, along with the hemoprotein cytochrome b5 (cyt b5), mediates electron transfer from NADH to fatty acid desaturases, P450 oxidases, methemoglobin, and ascorbyl free radical. In addition, b5R and cyt b5 can directly catalyze the reduction of hydroxylamine and amidoxime metabolites. This unit provides protocols for measuring the activity and mRNA expression of the cytochrome b5/cytochrome b5 reductase pathway, and for obtaining heterologous expression and purification of the soluble forms of each protein.
- Cytochrome b5 reductase;
- cytochrome b5;
- ferrihemoglobin reduction;
- protein purification