UNIT 4.23 Measurement of Soluble Epoxide Hydrolase (sEH) Activity
Published Online: 1 AUG 2007
Copyright © 2007 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Toxicology
How to Cite
Morisseau, C. and Hammock, B. D. 2007. Measurement of Soluble Epoxide Hydrolase (sEH) Activity. Current Protocols in Toxicology. 33:4.23:4.23.1–4.23.18.
- Published Online: 1 AUG 2007
- Published Print: AUG 2007
The human soluble epoxide hydrolase (sEH; EC 220.127.116.11) is the product of the EXPH2 gene. The sEH catalyzes the addition of a water molecule to an epoxide, resulting in the corresponding diol. Early work suggested a role of sEH in detoxifying a wide array of xenobiotic epoxides; however, recent findings clearly implicate the sEH in the regulation of blood pressure, pain, and inflammation through the hydrolysis of endogenous epoxy fatty acids such as epoxyeicosatrienoic acids (EETs). Both expression and activity of sEH are influenced by a wide array of xenobiotics, underlying how environmental contaminants could influence human health through sEH. This unit describes radiometric, fluorimetric, and mass spectrometric assays for measuring the activity of sEH and its inhibition. Curr. Protoc. Toxicol. 33:4.23.1-4.23.18. © 2007 by John Wiley & Sons, Inc.
- epoxyeicosatrienoic acids;
- blood pressure;